> Dear All, > > We have been sampling fish larvae for about five years and we have now come to > the attention of Animal Ethics bureaucrats who are concerned about how we are > killing our fish larvae. We cannot use formalin in our research centre for > health reasons and, since we need to look at otoliths of some of the larvae, we > usually throw larvae <10 mm in lenghth straight into 95% ethanol. My experience > is that the larvae die within a second or so and surely this must be as'humane' > as any method. Juveniles and adults are anaesthetised first before being put > into alcohol. The bureaucrats have queried the use of ethanol and put me under > pressure to justify this method. Can anyone out there point me to 'official' > guidelines for the 'humane' killing of fish larvae? > > Yours in hope and frustration, Paul. > > Dr Paul Humphries
I have no reference for you but I support your dropping fish larvae into alcohol as a 'humane' method of killing them.
I've killed a lot of fish larvae. I usually drop them in ethanol. One second and that's it. They're dead. It seems like a perfectly good and 'humane' way to do it to me.
A method suggested to me once was to put them in the freezer and let them cool down. Seems more like torture to me than a humane method.
Nick Tolimieri University of Auckland Leigh Marine Laboratory P.O. Box 349 Warkworth New Zealand
[log in to unmask]
Ph: 64 9 422 6111 Fax: 64 9 422 6113
><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> To leave the Fish-Sci list, Send blank message to: mailto:[log in to unmask] For information send INFO FISH-SCI to [log in to unmask] ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><>
|