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Recent discussion that lipofuscin based ageing of crustaceans is
burdensome may not be correct entirely. Marine chemist R. Harvey and his
student have developed a procedure for measuring extracted lipofuscin in
blue crabs using a rapid spectrophotometric assay. We've calibrated
standardized-lipofuscin measures through the first 2 years of life. The
technique is rapid and relatively inexpensive; we've been able to take
measures for moderately large sample sizes (n>500).
Ref:
Ju, S-J, D.H. Secor, and H.R. Harvey. 1999. Use of extractable
lipofuscin for age determination of blue crab Callinectes sapidus. Mar.
Ecol. Progr. Ser. 185: 171-179.
Regards,
David H. Secor, Ph.D.
Chesapeake Biological Laboratory, PO Box 38, Solomons, MD 20688
http://www.cbl.umces.edu/~secor/welcome.html
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