Hello everybody,

I am doing physiology experiments with African catfish cannulated in the
dorsal aorta; we are not 100% sure we are hitting the dorsal aorta but
after cannulation we do get blood. But... varying from 1 to sometimes 3
days the cannula gets blocked. I really don't have a clue what is the
reason as some fish give blood for over 4 days. As an anticoagulant I use
SodiumCitrate 1%. Can anybody help me with useful information?
Thanks a lot!

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Richard van Heeswijk,
Institute of Evolutionary & Ecological Sciences, Integrative Zoology
Kaiserstraat 63, 2300 RA, Leiden, the Netherlands,
tel:0715274262 fax:0715272490
e-mail:[log in to unmask]
http://wwwbio.leidenuniv.nl/~eew/G4/index.html
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