LISTSERV mailing list manager LISTSERV 15.5

Help for FISH-SCI Archives

FISH-SCI Archives

FISH-SCI Archives


Next Message | Previous Message
Next in Topic | Previous in Topic
Next by Same Author | Previous by Same Author
Chronologically | Most Recent First
Proportional Font | Monospaced Font


Join or Leave FISH-SCI
Reply | Post New Message
Search Archives

Subject: PCR Primers
From: Myke Bon <[log in to unmask]>
Reply-To:Scientific forum on fish and fisheries <[log in to unmask]>
Date:Wed, 7 Feb 2001 22:19:11 -0900

text/plain (28 lines)

Greetings Fish People,
I'm doing research on Lamprey in Alaska and at the point now that I
need to perform PCR on the DNA I do have for L. tridentada.  The
problem I have is that I need to determine a primer to use for the
process.  I've been to the Gene Bank though all information there is
mitochondrial; I'm working with genomic DNA.

We've been told it would be ideal to use a 10mer primer for
polymorphism research though most literature I've found suggests
between 18-29 for PCR.  So my question is as follows:

1)Can someone suggest how I can determine a primer for a species that I
dont know the Genomic Map on?  We have access to mitochondrial map of
L. fluviatis, It has been recommended we use it for a guideline to work
with L. tridentada, though we can't use it for genomic work I think.
Is there a universal primer (such as 'alu')that can work?  Any
suggestions will be recieved with great enthusiasm.

Thanks in advance!

Myke Bon

><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><>
       To leave the Fish-Sci list, Send blank message to:
        mailto:[log in to unmask]
Vacation? send SET FISH-SCI NOMAIL to [log in to unmask]
><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><>

Back to: Top of Message | Previous Page | Main FISH-SCI Page



CataList Email List Search Powered by the LISTSERV Email List Manager