|
Perhaps you could start with some universal primers that are known to work
for other cartilaginous species (ie., sharks, rays). Once you get some
fragments amplified and sequenced, you could then develop more specific
primers to that family. Good luck.
Myke Bon wrote:
> Greetings Fish People,
> I'm doing research on Lamprey in Alaska and at the point now that I
> need to perform PCR on the DNA I do have for L. tridentada. The
> problem I have is that I need to determine a primer to use for the
> process. I've been to the Gene Bank though all information there is
> mitochondrial; I'm working with genomic DNA.
>
> We've been told it would be ideal to use a 10mer primer for
> polymorphism research though most literature I've found suggests
> between 18-29 for PCR. So my question is as follows:
>
> 1)Can someone suggest how I can determine a primer for a species that I
> dont know the Genomic Map on? We have access to mitochondrial map of
> L. fluviatis, It has been recommended we use it for a guideline to work
> with L. tridentada, though we can't use it for genomic work I think.
> Is there a universal primer (such as 'alu')that can work? Any
> suggestions will be recieved with great enthusiasm.
>
> Thanks in advance!
>
> Myke Bon
>
> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><>
> To leave the Fish-Sci list, Send blank message to:
> mailto:[log in to unmask]
> Vacation? send SET FISH-SCI NOMAIL to [log in to unmask]
> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><>
--
***********************************
Trey Knott
Forensic Biologist
Center for Coastal Environmental Health
and Biomolecular Research
NOAA / National Ocean Service
219 Ft. Johnson Rd.
Charleston, SC 29412
phone: 843.762.8621
fax: 843.762.8700
email: [log in to unmask]
***********************************
><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><>
To leave the Fish-Sci list, Send blank message to:
mailto:[log in to unmask]
Vacation? send SET FISH-SCI NOMAIL to [log in to unmask]
><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><>
|
