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Greetings Fish People,
I'm doing research on Lamprey in Alaska and at the point now that I
need to perform PCR on the DNA I do have for L. tridentada. The
problem I have is that I need to determine a primer to use for the
process. I've been to the Gene Bank though all information there is
mitochondrial; I'm working with genomic DNA.
We've been told it would be ideal to use a 10mer primer for
polymorphism research though most literature I've found suggests
between 18-29 for PCR. So my question is as follows:
1)Can someone suggest how I can determine a primer for a species that I
dont know the Genomic Map on? We have access to mitochondrial map of
L. fluviatis, It has been recommended we use it for a guideline to work
with L. tridentada, though we can't use it for genomic work I think.
Is there a universal primer (such as 'alu')that can work? Any
suggestions will be recieved with great enthusiasm.
Thanks in advance!
Myke Bon
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