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Dr Harrod,
As an electrofishing instructor for the US Fish and Wildlife Service, I have always advised our class participants to use continuously-circulating tanks instead of closed systems. You can get submersible, battery-powered water pumps that will provide a steady flow of freshwater at ambient temperature and oxygen levels from the water body you are sampling. In my experience, this is far superior to the situation with a closed system where you try to aerate the water but also have to deal with increasing temp in the tank on warm days.
I urge you to consider using a water pump to run water through your tank and avoid the problems related to aeration altogether. --jbr
Dr. James B. Reynolds Emeritus Professor of Fisheries School of Fisheries and Ocean Sciences University of Alaska Fairbanks P.O. Box 757220 Fairbanks, Alaska 99775
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