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Fish folk -
Following is a summary of the responses received to my posting regarding measuring electrofishing effort. Thanks to all who contributed.
Cheers - Bruce
Electrofishing Effort – Responses posted to the Fish_Sci List, Dec. ‘03
Original posting:
We are attempting to create a standard for electrofishing effort. The fishing takes place with a Smith-Root Model 15D generator powered unit on small (>2 m wide, > 1.5 m depth) Canadian Shield streams (low conductivity, >60 ms/c) with a target species of brook trout. Assuming we get the power right how should we go about measuring effort? The idea is to create a standard to allow different crews to put the same amount of effort into electrofishing similar sites for the same target species.
Should the standard be the number of seconds that the anode is held over a square metre of substrate? This method might under-sample complex habitats (root wads) or over-sample others (bedrock bottom) if a standard of, say, 8 sec/m-2 was used.
Would it be better to use the length of time it takes to fish the station (often a 40 m length of stream)? Could another electrofisher operator sample the station in another season using a simple overall time figure?
Your thoughts/recommendations much appreciated. I will post a summary of responses at a later date if anyone is interested.
Bruce Thacker Senior Research Technician Centre for Northern Forest Ecosystem Reseach Ministry of Natural Resources 955 Oliver Road Thunder Bay, Ontario P7B 5E1 Canada
Tel.: 807-343-4015 Fax: 807-343-4001
Responses:
In British Columbia we have been standardizing electrofishing effort for depletion surveys in similar streams.
The concept now favoured is to keep the number of seconds of electrofishing (power on) the same for each pass of the sample reach. Thus there is no attempt to keep the effort constant on each square meter of habitat, which as you indicate would be counterproductive. It is hard to get electrofishing crews to stick to equal fishing effort on repeated passes, as when the fish really are depleted, it means more and more minutes of not catching anything.
I feel that it is not of overwhelming importance to get exactly replicated fishing times as other (environmental) factors affect your results more. These include: • Was the site disturbed by technicians before the fishing started? Walking through the sample reach drives most of the fish into refuges where they may well not emerge when electrofished. Of course the first electrofishing pass greatly disturbs the fish and subsequent passes will recover fewer fish. • The complexity of the habitat. Fish hiding in root wads and under boulders may be zapped in place or even killed without emerging. • Leaking stop nets. I haven't yet seen a stream with significant flow that is completely stopped off with nets. Typically the last fish caught in depletion surveys are near the stop nets, and likely are new immigrants to the reach.
Allen S. Gottesfeld Head Scientist Gitxsan and Wet'suwet'en Watershed Authorities PO Box 229, (1650 Omineca Street) Hazelton, B.C. V0J 1Y0 [log in to unmask] Phone: 250 849-5649 250 842-6780 local 328 Fax: 250 849-5648 250 842-6709
Bruce: I agree with the BC contingent--way too many variables to get a statistically meaningful standard measure of abundance in these north shore stream types. Especially with the two pass technique, disturbance in such small streams is inevitably going to influence the results. And the year to year variability (low flow years or seasons) in this part of the country makes it very difficult to standardize the assessment protocol--do we sample on a particular date each year, a rainfall event, a critical flow/temperature parameter, a particular month et al??
For the past ten years Grand Portage, along with the USFWS, has sampled 3 Lake Superior tributaries for bkt (coasters) and other salmonids. We have set 2 to 3 standard stations (300' or ~90 meters) for each stream below barriers. We do record the amount of seconds spent on each station but do not use it to evaluate abundance. We use it to generally correlate effort but it appears to have little meaning for the integrity of the stream and/or fish abundance. We merely sample each station length to see if we have a bimodal length distribution of YOY--perhaps indicating stocked vs. natural recruitment (Although, we often find tri- and quad- modal length distributions of rbt YOY, which are not stocked, only natural.)--and record the presence of pre-cocials and adults.
To simulate a standardized approach, our sampling efforts for these stations takes place in late July - early August (low flow when YOY have reached max. ? growth). We graphically represent our data as strict abundance year to year. It seems to give us a local index of population (?) integrity.
We also sample in late October-early November (fall rains w/ substantial rise in creek flow) but merely for presence absence of spawning male and females. This last year, for example, was dry (flow rates were low/water temps were high) and the fall rains (inches) were limited. We did not document a spawning run of bkt. We assume that they then spawn in the Lake. Based upon a twice per month sampling effort after ice out until ice formation, we also notice a significant emptying of YOY in the streams. On a monthly basis, overall abundance appears to be equal during the ice-free period.
We use a back-pack shocker made at the University of Wisconsin-Madison Engineering Department. It is set-up with Quadra pulse: we seldom use it, but probably should as it is supposed prevent tetany in these smaller salmonids. Our settings are regularly: 68-81 pps rate; 380-400 volts; 14.4% duty cycle. In relatively low conductivity waters, >200microSiemens, our depth and width parameters are similar to yours.
Hope these thoughts help...I would be interested to see what you come up with; perhaps there is a statistically meaningful protocol to sample these streams. Let me know... Ben
Benjamin Whiting, Fish and Wildlife Biologist Grand Portage Band of Chippewa PO Box 428 27 Store Road Grand Portage, MN 55605 [log in to unmask] 218-475-2415 ext. 22 -2615fax -2695home
We have run into similar problems, in establishing index sites or doing comparisons between systems or between years. The standard would be to enclose sites (stop nets at both ends) and do a three pass removal method or mark recapture. Both have flaws.
The three pass method requires equal effort (seconds on each pass) however, if the fish populations are high, considerably more time is spent "catching" fish on the first pass and more time is spent looking for fish on the last pass. This could result in a small error in the estimated population. (I do not place much emphasis on the time factor). It is much more important to capture a high percentage of the fish. Using the mark recapture, (which if done properly should be more accurate than the removal method) you should give the marked fish a fairly significant amount of time to re distribute. In many instances this is not practical.
By using time as a basis for CPUE a number of fairly significant variables are introduced, as you have mentioned which will make doing comparisons difficult. Some operators are better than others, some are faster, some are slower, the assistants that are helping net the fish play a significant role and can dramatically change the time required to cover an area and capture efficiency. I prefer to make comparison on a per unit area basis, which should eliminate a lot of these variables. If you do a three pass removal method using stop nets (you need to spend some time on these, particularly the downstream net). The estimated populations should be consistent. More efficient crews should have better confidence intervals in the population estimates but at least there is a basis for comparisons.
One factor that I have found very important is site selection. Try to avoid sites that are difficult to electrofish. Very deep pools, large root wads and deeply undercut banks make life much more difficult and results more variable. Try to find areas that are representative of the carrying capacity of the reach (based on professional judgement) but that can be electrofished effectively. A site with a large number of "small cover components" can be sampled much more effectively and consistently than a site with one large piece of cover.
Another option would be to sample habitat units ie riffles vs riffles and pools vs pools. The only problem with this method is that in small streams habitat units can be short and you could scare fish out of the area while putting in the stop nets. (Bottom net should go in first- my experience is that fish that are disturbed while putting in the net will only go a short distance upstream and will still remain in the site but downstream travel could take them out of the site.) Or you could sample a sequence of habitat units ie your sites should contain a pool riffle glide sequence. The interface between habitat units can be key fish holding areas depending on the species.
Placement of stop nets is important. Wide, shallow, low velocity and small substrate areas are preferred. If you put stop nets in that fall down or have significant "leaks" you are only fooling yourself. Even the most carefully placed nets are still "leaky" but if you do a good job you can reduce the problems. Try not to make the downstream net a nice place to run and hide. (lean the top of the net downstream so that it does not provide shade and overhead cover and so fish will accumulate away from the spot where the net hits the stream bottom –i.e.,. use a deep net).
Some sort of consistency in site selection is probably more important than the electrofishing method.
I have found also that most species of fry tend to concentrate in shallow low velocity shoreline areas. So in wider sections of stream the density estimates will decrease despite the fact that the number of fry per length of stream remains the same and probably the density in the suitable habitat is consistent as well. The same is not necessarily true for older or larger age classes. So it depends on what you are trying to measure. It would be preferable to document fish numbers on the basis of estimated number per unit area and per unit length just to be on the safe site.
There has also been some work done looking at macro habitats ie small habitat specific sites, with the same depth velocity and cover components however I have little experience with those methods.
If you are not using stop nets, then it becomes much more difficult to get consistency. You go from herding to hunting and differences in crew efficiency would probably be magnified. Although with good crews I would still prefer number of fish captured per unit area or length rather than time. Record all three and you can compare to see which gives you the most consistent results.
B.G. Blackman R.P. Bio Senior Fish Biologist Peace/Williston Fish & Wildlife Compensation Program 1011 Fourth Avenue, 3rd. Floor Prince George, BC V2L 3H9 Phone: (250) 565 - 6413 Fax: (250) 565 - 6629 Email: [log in to unmask] Website: http://www.bchydro.com/pwcp <http://www.bchydro.com/pwcp>
I agree with Allen Gottesfeld, with backpack shockers, it is better to use a fixed time as your measure of effort (one pass), rather than try to standardize for each square meter. Another variation of standard effort with backpack shockers is to cover a fixed distance for each pass. Time as an effort unit has the advantage of being easy to measure; distance has the advantage of allowing for increased catches if more fish are present. Either way, the crew knows how much sampling is required before stopping. Also, you made an important point in your original inquiry: "Assuming we get the power right..."; this is a key aspect of standardizing electrofishing effort and it is worth the preliminary work to set up a standardized power table for your operation so that you can adjust for the effects of water conductivity, a key factor that varies even in Canadian Shield streams. Water conductivity is the single most important habitat factor affecting electrofishing efficiency.
Jim Reynolds, Peace Corps Fiji Institute of Applied Sciences University of the South Pacific Suva, Fiji Islands
Bruce,
FYI. Below are some comments on electrofishing effort from Scott Stranko, who does a large amount of backpack electrofishing in Maryland streams.
regards, Erik Zlokovitz Maryland Dept. of Natural Resources-Fisheries Service Tawes State Office Building, C-2 580 Taylor Ave. Annapolis, MD 21401 [log in to unmask] 410-260-8306
-----Original Message----- From: Stranko, Scott Sent: Tuesday, December 23, 2003 2:12 PM To: Zlokovitz, Erik Cc: Millard, Chris Subject: RE: electrofishing effort
Thanks Erik.
Catch per unit of effort in time is a commonly used measure of effort for electrofishing. An example is # of fish/second. Distance is also used. As you know we use a standard length of stream with the MBSS (75 m). However, we use one anode for every 2 - 3 m of stream width to be sure we are covering the habitat effectively. We also keep track of the seconds shocked by each anode so that we can generate a catch per unit of effort in seconds for our electrofishing surveys. So we have a standard length of stream plus total seconds shocked by anodes so we can standardize time too.
Smith Root electrofishers use something called P.O.W. (programmable, output, waveforms). POW uses direct current output that can be set to pulse at whatever interval you may want. I think the most recent version of the model 15 electrofisher is capable of about 250 different possible pulse output settings. These settings allow effective electrofishing in a wide range of conductivity conditions and can be set to cause the least possible injury to the fish. It is important to record the POW setting, voltage, and conductivity of the water while electrofishing so that your effectiveness is documented.
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