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Dear Dr. Harrod,
I am in the middle of my PhD and have been looking at morphology in
sticklebacks. My fish were anaethetised (they were being weighed and
measured at the same time) and then laid out on sheet of acetate graph
paper (the stuff with tiny squares) so that I had both a transverse and
longitudinal scale. I used a fairly cheap digital camera which I fixed the
aperture & shutter speed on. It was fixed on a tripod above the specimen.
Illumination was from 2 desk lamps angled at 45 degrees on to the fish. Are
you doing colouration analysis too ? This makes the light levels more
important and you have to remember to put in a colour standard. I have
found ImageJ software (free off the net) is quite good for morphometrics,
as is TPSDig.
Apologies if this is stuff you know already !
Claire Inness
=====================================
Claire Inness
Fish Biology Group
Division of Environmental and Evolutionary Biology
Graham Kerr Building
University of Glasgow
Glasgow
Scotland, UK
G12 8QQ
Tel: + 44 0141 330 3534
Omit zero if dialing from outside the UK.
email: [log in to unmask]
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