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From my experiences with fish suppliers, it would be highly unlikely that the fish were "clean". If you ever go into a supplier’s facility, the fish are back into tanks in numbers that would astound you. I would de-parasitize all the fish before you infect them, just to be safe.
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Anthony I. Mazeroll, Ph.D.
Professor of Biology
Soka University of America
No human being, however great, or powerful, was ever so free as a FISH.
John Ruskin (1819–1900), English art critic
----- Original Message ----
From: Michael Robinson <[log in to unmask]>
To: [log in to unmask]
Sent: Tuesday, March 25, 2008 10:53:45 AM
Subject: experimentally parasitize small fish?
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Dear list,
I am looking for information on ways to experimentally parasitize small
freshwater fish. I am not too concerned about the type of parasite, as long as
I can collect them afterwards. I have found some information (see below), but I
was hoping someone would have some advice or experiences that they wouldn't mind
sharing.
Thank you very much!
Mike
What I am thinking and what I have found:
I want to parasitize small, readily available freshwater fish. I am thinking of
either guppies (Poecilia reticulata) or zebrafish (Danio rerio). I have to have
a non-parasitized control group (no parasites at all), so I figured I could get
all fish from a supplier so they were clean of ALL parasites and then infect
one half of them. Is it reasonable that I can get non-parasitized fish from a
supplier?
It looks like I can parasitize the fish with Gyrodactylus (a monogean) either by
placing the parasite on the fish directly or housing parasitized fish with
non-parasitized fish. I have a number of references on this already. I get
the impression that these worms are easy to obtain from fish stores and
possibly from the wild. Is that true?
For a variety of reasons, I would prefer to use Camallanus (or a similar
gastrointestinal nematode parasite). I know that McMinn (1990) experimentally
infested guppies with Camallanus, but I’m not quite sure how he did it. Can
anyone shed light on this for me? Does anyone know where I can find him?
McMinn, H. (1990) Effects of the nematode parasite Camallanus cotti on sexual
and nonsexual behaviors in the guppy (Poecilia reticulata). Am. Zool. 30(2):
245-249
Again, thank you all very much for your time!
_______________________________________________________________________________
Michael P. Robinson
Dept. of Biology University of Miami
P.O. Box 249118 Coral Gables FL 33124-0421
[log in to unmask] web: www.bio.miami.edu/robinson
305-284-4313 fax: 305-284-3039
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