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I have a student who's trying to clear and stain a wrasse (genus
Cirrhilabrus) for a class project. Other students have had good success
using the same protocol (adapted from Cailliet, Love, and Ebeling) on
cyprinids and centrachids but her fish isn't picking up any color from the
final stain (we're using 1% Alizarin red in KOH), though it's been soaking
for a week. I'm wondering if this could be because it's a labrid. (E.g., do
they need a xylene soak to remove excess lipids, as a salmonid does?) Does
anyone on the list have any advice or experience clearing and staining
marine fishes?
Thanks,
Duncan Elkins
University of Georgia
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