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Subject: Re: Treatment of samples for PCB/Dioxin analyses.
From: "West, James E (DFW)" <[log in to unmask]>
Reply-To:Scientific forum on fish and fisheries <[log in to unmask]>
Date:Mon, 1 Dec 2008 11:08:17 -0800

text/plain (58 lines)

Chris, we have been monitoring PCBs and other organics in fish tissues for almost 20 years and our protocols specify -20C.   You are correct however that fatty acids require -80C asap, and it is best to keep them in the whole body form (frozen of course)prior to homogenization.

James E. West
Washington Department of Fish and Wildlife
Marine Resources Division
600 Capitol Way N
Olympia, WA 98501
v1 360.902.2842
v2 206.302.2427
fax 360.902.2944
[log in to unmask]
Puget Sound Assessment and Monitoring Program

-----Original Message-----
From: Scientific forum on fish and fisheries [mailto:[log in to unmask]] On Behalf Of Chris Harrod
Sent: Thursday, November 27, 2008 6:45 AM
To: [log in to unmask]
Subject: Treatment of samples for PCB/Dioxin analyses.


I'm hoping that the Fish-Sci community can give me the benefit of their expertise.

I have been asked to provide some fish samples for subsequent analyses of PCBs and Polychlorinated dibenzodioxins (dioxins).
I had the impression that samples, once taken, should be stored at -80C to prevent loss of labile compounds.
I follow this approach for fatty acid work for example, and it seems to make sense to me for any compounds that are likely to be associated with lipids.

However, one of the labs I have contacted regarding the analyses clearly stated that freezing at -20C was sufficient.

Comments/pointers to literature/procedures etc much appreciated!

All the best from an almost sunny Belfast


><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><
Dr Chris Harrod
Lecturer in Fish and Aquatic Ecology
Queen's University Belfast
School of Biological Sciences
Medical Biology Centre
97 Lisburn Road
Belfast BT9 7BL
[log in to unmask]
Tel: +44 (0) 28 909 72271
Mob: +44 (0) 79 77419314
Lab: +44 (0) 28 909 72106
Fax: +44 (0) 28 909 75877
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