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Just about anything you do to a fish stresses them (stress being defined as
increased cortisol levels). Capture, netting to transfer, placing in a
strange environment (such as a pail), and anesthesia induction (un-buffered
MS-222 and methylpentynol for sure and I suspect the others as well)are all
stressors. Presumably, they could all be additive. It might be better for
the fish to simply measure, tag and return them directly to their habitat. I
can't speak to the collecting, which obviously can be a big stressor (if I
am wielding the handnet, bet on the fish) but measuring and tagging can be
done very quickly.
I have tried to collect with MS-222 and quinaldine with very limited
success. Unless your critters hole-up or are micro-rheotactic the dilution
factor seems to be overwhelming,
Richard E. Spieler, PhD
Professor and Director of
Academic Programs.
Oceanographic Center
Nova Southeastern University
8000 N Ocean Drive
Dania Beach, FL 33004
Telephone (954) 262 3613
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-----Original Message-----
From: Scientific forum on fish and fisheries
[mailto:[log in to unmask]] On Behalf Of Sprague, Gary
Sent: Friday, March 05, 2010 9:41 AM
To: [log in to unmask]
Subject: Re: Anaesthetic for intertidal fish
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Hello,
Looking at an old copy of Trout and Salmon Culture (California Fish Bulletin
Number 164 -1980) it identifies that the following have been used in the
past as fish anesthetics:
Creson, ether, carbon dioxide, chlorotone (Chlorobutanol), sodium amytal,
methyl pentynol, quinaldine, MS-222.
I have seen alcohol used in one situation, but I do not remember the type.
Gary
Gary Sprague
206.233-5108
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-----Original Message-----
From: Scientific forum on fish and fisheries
[mailto:[log in to unmask]] On Behalf Of Magnus Johnson
Sent: Thursday, March 04, 2010 11:22 AM
To: [log in to unmask]
Subject: Anaesthetic for intertidal fish
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Hi All,
We are doing a project on intertidal fish and want to knock them out so that
Chris can tag and measure them with minimal stress to the fish.
I've used clove oil in the past (cheap and easy to get hold of) but have
recently been told by a home office inspector that this went out with the
dinosaurs!
I've also been told that in the UK it is ok to use clove oil or
2-phexyethanol to kill fish but not to anaesthetise and bring round and that
we should use MS222 (Tricaine methanesulfonate) instead.
Can anyone offer some advice/suggestions?
Cheers, Magnus
_____________________________________________________________
Dr Magnus L. Johnson
Centre for Environmental and Marine Sciences University of Hull
http://www.hull.ac.uk/cems
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