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Dear all,
we are trying to extract DNA for PCR amplification from fish eggs (a damselfish). Since the eggs are often embedded in the sticky mucus used by the female to lay them on the rock nests, we would like to find some form of chemical or enzyme that can be used to "wash" the eggs and remove the traces of mucus that can interfere with the isolation of clean DNA.
Any tips are welcome.

Thanks!

Stefano.
________________________________________
Dr Stefano Mariani
MARine Biodiversity, Ecology & Evolution
UCD School of Biology & Environmental Science
Science and Education Research Centre (West)
University College Dublin
Belfield
Dublin 4
Republic of Ireland
tel. +353.1.716.2347
fax. +353.1.716.1153
http://www.ucd.ie/marbee/stefano_mariani.html

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