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Stefano,

This is from a co-worker(Emily Chandler) who knows way more than I about DNA

> There are lots of things to try.  My snails were really slimy but 
> surprisingly I found that the Qiagen DNA kit was the best in getting 
> rid of the mucus.  I don't have the procedure here, but if you search 
> through the mollusk literature, there are several other recommended 
> protocols for getting rid of mucus. 

Cheers,

Ben

==================================================
Benjamin Galuardi
Research Scientist
Large Pelagics Research Center
UMass Amherst and Marine Fisheries Inst
932 Washington Street
Gloucester, MA 01930
(978) 283-0368 lab
==================================================


On 11/9/2011 9:44 AM, stefano mariani wrote:
>> <>   ><>   ><>   ><>   ><>   ><>   ><>   ><>   ><>   ><>   ><>   ><>   ><>
>             REPLIES WILL BE SENT TO THE FISH-SCI LIST
> <><   <><   <><   <><   <><   <><   <><   <><   <><   <><   <><   <><   <><
>
> Dear all,
> we are trying to extract DNA for PCR amplification from fish eggs (a damselfish). Since the eggs are often embedded in the sticky mucus used by the female to lay them on the rock nests, we would like to find some form of chemical or enzyme that can be used to "wash" the eggs and remove the traces of mucus that can interfere with the isolation of clean DNA.
> Any tips are welcome.
>
> Thanks!
>
> Stefano.
> ________________________________________
> Dr Stefano Mariani
> MARine Biodiversity, Ecology&  Evolution
> UCD School of Biology&  Environmental Science
> Science and Education Research Centre (West)
> University College Dublin
> Belfield
> Dublin 4
> Republic of Ireland
> tel. +353.1.716.2347
> fax. +353.1.716.1153
> http://www.ucd.ie/marbee/stefano_mariani.html
>
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