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Subject: Re: Fixative/preservative for gonads
From: Valerio Visconti <[log in to unmask]>
Reply-To:Scientific forum on fish and fisheries <[log in to unmask]>
Date:Sat, 30 Jul 2016 07:02:58 +0200
Content-Type:text/plain
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Hi Chris,

I have recently used FAAC for both tropical and temperate fish gonads
samples with very good results.

Prepare as below (to make 1L):
-100mL Formalin (37-40%);
-50mL Glacial Acetic Acid;
-850ml de-ionised water;
-13g Calcium Chloride (CaCl).

I have left my samples in this fixative for six months before histology
without problems. However, it is recommended to move the samples to 70%
ethanol.

Cheers

Valerio

On 29/07/2016 9:35 pm, "Chris Harrod" <[log in to unmask]> wrote:

> ><>  ><>  ><>  ><>  ><>  ><>  ><>  ><>  ><>  ><>  ><>  ><>  ><>
>            REPLIES WILL BE SENT TO THE FISH-SCI LIST
> <><  <><  <><  <><  <><  <><  <><  <><  <><  <><  <><  <><  <><
>
> Folks
>
> After almost 20 years, I'm going to be returning to some work on fish
> reproductive investment.
> In my PhD I used Bouin's fixative to fix and store fish gonads prior to
> doing egg counts, measurements etc., but am looking for a less toxic option
> for our new work.
> I imagine things have moved on a bit in the last 2 decades  - have they?
> Can anyone recommend chemicals that aren't going to cause health and safety
> nightmares?
>
> Cheers
>
> Chris
>
>
> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><> ><>
> Professor Chris Harrod
> (Fish & Aquatic Ecology)
> Email: [log in to unmask]
> Twitter: @chris_harrod
> www.harrodlab.net
> HarrodLab: Fish and Stable Isotope Ecology Laboratory
> Instituto de Ciencias Naturales Alexander von Humboldt,
> Universidad de Antofagasta,
> Avenida Angamos 601, Antofagasta, Chile
> Chile Mobile: +56 9 7399 7792
> Chile Office: +56 55 2637400
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