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Dear All,
While the above topic is being discussed by the group, I was
wondering if any one had any thoughts on a problem I have.
I work on sperm in the guppy (Poecilia reticulata) and I would
like to bring some preserved females across from Trinidad. What I'm looking
for is a method of killing and preserving them such that the sperm in the
ovarian cavity of the female remains undisturbed. Ideally the preserving fluid
would stop all cellular action immediately. I would like the female to reach
me (in Scotland) with her ovarian cavity (and any sperm present) in
exactly the same condition as when she was caught.
I would be most grateful for any ideas that you may have on
suitable methods of treatment, and will be happy to pass these on to any
one else interested.
Yours sincerely,
Iain Matthews,
School of Biological and Medical Sciences,
University of St Andrews,
St Andrews,
Fife. Scotland.
Tel + 44 1334 476161 Ext. 3220
Fax + 44 1334 463600
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