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Subject:

Re: Information on RNA/DNA ratios

From:

Graham Pierce <[log in to unmask]>

Reply-To:

Academic forum on fisheries ecology and related topics <[log in to unmask]>

Date:

Wed, 18 Dec 1996 11:33:48 +0000

Content-Type:

text/plain

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> Dear Graham
> I hope everything goes well in Aberdeen and I was
> glad to know that your proposal was funded by FAIR. We
> did not have the same luck with our proposal on
> reproduction abd there are no prospects for funding in cephalopods
> for the next year (or more ...). Nevertheles, I had
> a national project (PRAXIS XXI) funded and I will return to
> the ichthyology for the next three years.
> I would like to ask your help on the following subject:
> I have a MsC student working in a local park with
> dolphins (T. truncatus) that is going to study the role
> of the feeding regime on the growth and condition of
> several animals (8). These animals are observed at regular
> intervals and samples of urine, blood and feces are colected
> on a montlhy basis. My question is: is it possible to use
> the blood samples to extract the RNA and DNA to use the
> respective ratios? Do you know any information that might
> have been published on this subject or anyone that is
> (or has) working in this subject?

Hello Pedro,

I will ask the person who did the work for us, Karl Siegert. I suspect it
is not possible from blood samples but I could be wrong, will let you know.

I have a related question. If you are able to study different feeding regimes in
dolphins, is there any possibility to collect dolphin faeces/regurgitates, e.g.
from the bottom of the pool. I am interested in whether cephalopod beaks are
defecated or regurgitated and how the time course for this process compares
to the passage time through the gut for otoliths.

> We are now trying to collect as much information as possible
> but my feeling (from preliminary searches) is that the information
> is limited.
> I look forward for your news on this subject.
> I will leave next friday to spent the Christmas and new year in
> Lisbon.
> I wish a good Christmas season and a happy 1997.
> Best regards
> J. Pedro Andrade

Best wishes and happy Christmas

Graham




>
>
================================================
Dr Graham J. Pierce

Lecturer in Fishery Science

Department of Zoology, University of Aberdeen,
Tillydrone Avenue, Aberdeen AB24 2TZ, UK
Phone 44 (0)1224 272459
Fax 44 (0)1224 272396
e-mail [log in to unmask]
WWW http://www.abdn.ac.uk/~nhi104/

AND

Scottish Office Agriculture, Environment
and Fisheries Department Marine Laboratory,
PO Box 101, Victoria Road, Aberdeen AB9 8DB, UK
Phone 44 (0)1224 295522 or 876544
Fax 44 (0)1224 295511
e-mail [log in to unmask]
================================================


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