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Subject: Re: Preservation of larval fish
From: David Lemarie <[log in to unmask]>
Reply-To:Scientific forum on fish and fisheries <[log in to unmask]>
Date:Wed, 21 Jan 1998 10:55:24 -0700

text/plain (29 lines)

     We have used the following preservative ("4CF-1G") for larval striped
     bass.  The tissue remained clear for long periods, allowing
     examination by light microscopy for presence of an inflated swim

     1.16 g NaH2PO4
     0.27 g NaOH
     88 mL water
     10 mL 40% formaldehyde
     2 mL 50% glutaraldehyde

     Refrigerate after preparation of stock.

     Prior to use, add 2% DMSO to assist in the rapid transfer of the
     fixative into the tissue.

     Reference:  McDowell, E. M. and B. F. Trump.  1976.  Histologic
     fixatives suitable for diagnostic light and electron microscopy.
     Arch. Pathol. Lab. Med., Vol. 100.

     David Lemarie
     USGS-Biological Resources Division
     Leetown Science Center
     1700 Leetown Road
     Kearneysville, WV  25430

     Phone:  304/725-8461 ext. 214
     E-mail:  [log in to unmask]

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