We have used the following preservative ("4CF-1G") for larval striped
     bass.  The tissue remained clear for long periods, allowing
     examination by light microscopy for presence of an inflated swim
     bladder.

     1.16 g NaH2PO4
     0.27 g NaOH
     88 mL water
     10 mL 40% formaldehyde
     2 mL 50% glutaraldehyde

     Refrigerate after preparation of stock.

     Prior to use, add 2% DMSO to assist in the rapid transfer of the
     fixative into the tissue.

     Reference:  McDowell, E. M. and B. F. Trump.  1976.  Histologic
     fixatives suitable for diagnostic light and electron microscopy.
     Arch. Pathol. Lab. Med., Vol. 100.


     David Lemarie
     USGS-Biological Resources Division
     Leetown Science Center
     1700 Leetown Road
     Kearneysville, WV  25430

     Phone:  304/725-8461 ext. 214
     E-mail:  [log in to unmask]