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Justin,
You may already have tried these elementary suggestions, but here goes anyway:
-- Brief immersion in Bouin's solution (1 minute) prior to immersion in 10%
buffered formalin.
-- Make up fresh buffered formalin if using old stock.
-- If feasible, consider using a tuberculin syringe to inject formalin into
the abdominal cavities of the fish (wear goggles!).
Good luck,
-- Jeff
Jeffrey C. Wolf, DVM, Dipl. ACVP
Department of Biomedical Sciences and Pathobiology
Virginia-Maryland Regional College of Veterinary Medicine
Blacksburg, Virginia USA 24061-0442
Tel:(540)231-6583
Fax:(540)231-7367
[log in to unmask]
***************
> Im currently working on a project involving larval fish and wish
>to preserve specimans for later observation. Can anyone suggest a good
>method by which to preserve these larvae? Ive tried formalin but there is
>still some amount of degradation and cloudiness in the tissue.
>
> Any suggestions?
>
>Gratefully,
>
> Justin Krebs
> Deptartment of Biological Sciences
> Florida Institute of Technology
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