Has anyone experienced toxicity problems while marking otoliths with alizarin complexone?
I recently immersed a batch of cichlid juveniles in an aerated freshwater solution of 0.2 gram/L Alizarin complexone. After 5 hours many of the fish were swimming erratically, rolling over onto their backs, etc. I performed repeated water changes over a couple hours. Most recovered witihin 24 hours, but I experienced ~10% mortality.
Any experienced Alizarin users with insights into what I may have done wrong is encouraged to reply! (solution too strong? immersion time too long?)
Note-Alizarin solution was made up fresh from a container of powder only 4 months old. Water was fresh and aged to remove chlorine.
Thanks!
J. Jasper Lament Department of Biology, University of Miami, Coral Gables, FL 33124-0421
Office Phone: (305) 284-4933 Fax: (305) 284-3039
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