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We have used the following preservative ("4CF-1G") for larval striped
bass. The tissue remained clear for long periods, allowing
examination by light microscopy for presence of an inflated swim
bladder.
1.16 g NaH2PO4
0.27 g NaOH
88 mL water
10 mL 40% formaldehyde
2 mL 50% glutaraldehyde
Refrigerate after preparation of stock.
Prior to use, add 2% DMSO to assist in the rapid transfer of the
fixative into the tissue.
Reference: McDowell, E. M. and B. F. Trump. 1976. Histologic
fixatives suitable for diagnostic light and electron microscopy.
Arch. Pathol. Lab. Med., Vol. 100.
David Lemarie
USGS-Biological Resources Division
Leetown Science Center
1700 Leetown Road
Kearneysville, WV 25430
Phone: 304/725-8461 ext. 214
E-mail: [log in to unmask]
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