Hello everyone. I recently conducted growth experiments on squid at two different temperatures. The squid which were placed into 15 degree Celsius water from an ambient of about 22 Celsius had a band in their statoliths (analagous to fish otoliths) which was obvious as a transparent band in incident light but almost invisible in transmitted light. Squid which went into 20 Celsius water in the lab (from 22 Celsius ambient) showed no similar band. There was a strongish check visible at the start of the band but the increments thereafter looked no different to those laid down before that check. I've looked in the squid statolith literature but the only reference to zones are the darker vs lighter ones seen commonly in squid statoliths and widely reported. I've looked in the fish literature too. I know that temperature can produce very visible checks in fish otoliths. And that different zones have been explained as due to metamorphosis or a habitat shift - again all seen in transmitted light. My squid were kept at a constant temperature in my experiment for about 100 days and the band is visible for about 1/3 of that period and is then less evident, but the optical properties are still tending more towards transparency vs opacity. Has anyone seen a similar band(s) in incident light in otoliths or statoliths? Can anyone offer any explanation? Might there be a chemical explanation in the way that calcium is deposited to have different optical properties (total stab in the dark here?!) Hope someone can offer some words of wisdom for me.
Thanks
Emma
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